Bikash Baral and V. N. P. Gupta
Biotechnology Unit, Central Department of Botany, Tribhuvan University, Kirtipur

Abstract

The Nitrogenous compounds translocated to the aerial parts from the nodules are in the form of ureides, allantoin and allantoic acid. The synthesis of ureides occurs mainly in root nodules via the coordination of the plant bacteria association. But still, the occurrence, localization, or properties of the enzymes involved in the assimilation of ureides in shoot tissues has not been resolved so far. We have examined the total amount of the ureides translocated to the aerial parts by the process of xylem bleedings method by taking the allantoin as internal standard as per the principles of Young and Conway. For the determination of ureides, two rhizobial isolates were isolated from Phaseolus vulgaris L. from Manang and Pisum sativum L. from Kathmandu valley. Other different parameters include the shoots length, roots length, nodules number, nodules biomass, plant biomass and the N of stem, leaves and nodules at 20, 30 and 40 days. The ANOVA result showed insignificant result (P> 0.05) in the different inoculums, shoot length, root length and the nodules nitrogen where it was Significant (P< 0.05) for both rhizobial isolates for nodulation. These ureides have been compared with the total Nitrogen detected from the nodules, stems and the leaves by the help of Micro- Kjeldahl method. The ANOVA result for ureides showed highly significant difference at 99% level of significance for both isolates (Treatment combinations=6.633, Bacterial Blocks= 3.502, P<0.01). The overall ureides concentration for KRI and MR2 was 335.854 mg/l and 338.575mg/l respectively, while total concentration of ureides for KRI and MR2 was 574.05mg/l and 501.075mg/l respectively at 45 days of plant sowing. The statistical examination of allantoin
equivalent also revealed that there was a positive correlation between treatment combinations and blocks.

Keywords: Allantoin, assimilation, nitrogenous compound, nodules, rhizobial isolates, ureides, translocation,