Ditya Lamichhaney1*, Bimala Pant2 , Suraj Baidya2 and Lalit Sah3
1 Himalayan College of Agricultural Sciences and Technology (HICAST), Kathmandu, Nepal
2 Nepal Agricultural Research Council (NARC), Lalitpur, Nepal
3 International Development Enterprises (iDE), Lalitpur,Nepal
*Corresponding author’s email: lamichhaneyditya1@gmail.com
* ORCID ID: https://orcid.org/0000-0002-5230-7579
Abstract
The study was conducted from July to October, 2019. The objective of this study was to identify viral diseases in vegetable pocket areas of Kathmandu, Bhaktapur and Lalitpur districts. Sample size of 102 households of vegetable cultivated areas was selected through purposive random sampling technique. Data were collected from subsistence and commercial vegetable farms using pre- tested semi- structured questionnaires. 30 plant samples showing virus like symptoms were further diagnosed using enzyme-linked immuno-sorbent assay (ELISA) and Immunostrip detection tests. Of total 30 vegetable crops samples suspected for prevalence of virus pathogen, 21 samples were detected with viral infection. This study provided laboratory test confirmation on presence of virus diseases, like Zucchini Yellow Mosaic Virus disease in pumpkin, sponge gourd and cucumber, Cucumber Mosaic Virus disease in chilli and tomato, Tomato Mosaic Virus disease in tomato, Turnip Mosaic Virus disease in broad leaf mustard, Chilli Veinal Mottle Virus disease in chilli, Squash Mosaic Virus disease in broad leaf mustard and Bean Common Mosaic Virus disease in common bean in specific locations of Kathmandu, Bhaktapur and Lalitpur districts. This study provided first report of Squash Mosaic Virus in Broad Leaf Mustard cultivated areas of Lalitpur district. The results obtained from this study can be used in developing location specific integrated management strategies for viral diseases.
Keywords: ACP ELISA, Antibody, Antigen, Vegetable crops, Virus disease detection tests