Tara L. Lama and Vilma D. Alejandro
Department of Agro-Hort. Science, Benguet State University, La Trinidad, Benguet, Philippines

DOI

ABSTRACT

Plant growth, number of shoots, nodes/shoot and shoot height varied with different shoot proliferation media. Shoot proliferation was best in Lama medium (Murashige and Skoog9 MS)+ 2.5 Gibberllic acid (GA3) + 10% coconut water). Clone 1-1039 gave significantly hiher number of shoots. Combination of plant growth regulators (PGR) in Dodds medium (MS + 0.4 ppm GA3 + 0.5 ppm Benzylamino purine (PAB) + 0.01 ppm Napthalene acetic acid (NAA) was equally effective as Lama medium in proliferating the shoots in in vitro. Dodds protocol (MS + 5 ppm BAP, 500 ppm of Cloroclorine chloride (CCC) and 8% sugar) affected higher number and weight of the microtubers. Presence of CCC and BAP in the fiber induction media showed synergistic effect on both tuber induction and bulking.